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KMID : 0359319940340030517
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Korean Journal of Veterinary Research
1994 Volume.34 No. 3 p.517 ~ p.527
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Characterization of canine coronaviruses isolated from the dogs with diarrhea in Korea
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박종현/Park, Jong Hyeon
허원/황의경/전무형/이병형/Huh, Won/Hwang, Eui Kyung/Jun, Moo Hyung/Lee, Byung Hyung
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Abstract
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An attempt was made to isolate a causative viral agents from the fecal specimens of the diseased dogs with the gastroenteritis symptoms. Two coronavirus-like agents were isolated by serial dilution end point method and plaque assay. The isolates were characterized in terms of cytopathology, antigenicity, replication, physicochemical and morphological properties.
The results obtained through the experiment were as follows;
1. Among 7 fecal specimens collected from the dogs with enteric disease, 2(28.6%)coronavirus-like agents showing typical cytopathic effects of canine coronavirus were isolated, and designated as CCV D1 and CCV D2, respectively.
2. By the cross-neutralization test and indirect immunofluoresence antibody test, the isolates were antigenically indentified as the standard CCV. The viruses were replicated only in the cytoplasm of A-72 cells.
3. The isolates showed no haemagglutinating activity against the erythrocytes from 11 kinds of animals.
4. The electron microscopic observation for the isolates showed spherical and pleomorphic features, covered with club-shaped projections on the surface. The size of particles was ranged from 70 to 150nm.
5. In one-step growth curve for the isolates in A-72 cells, maximum titers of intracellular vims was 10^(4.6) TCID_(50)/0.1㎖ at 46 hrs postinoculation(pi) of CCV Dl and 10^(4.4) TCID_(50)/0.1㎖ at 34 hrs pi of CCV D2. The maximum titers of extracellular virus was 10^(5.5) TCID_(50)/0.1㎖ at 58 hrs pi of CCV D1 and 10^(5.8) TCID_(50)/0.1㎖ at 46 hrs pi of CCV D2.
6. In physicochemical property test, the isolates were very sensitive to choroform and were found to be RNA virus. The viruses was stable at pH 3.0 for 1 hr and at 22℃ for 5 hrs. However, infectivity titers reduced remarkably by treatment with 56℃ for l0min.
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KEYWORD
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